American Society for Clinical Pathology (ASCP) Histotechnician Certification Practice Exam

Why is it important to prevent glycolysis in serum samples?

1. To maintain pH levels

2. To ensure accurate glucose measurements

3. To reduce viscosity

4. To enhance clotting

The correct answer is: To ensure accurate glucose measurements

Preventing glycolysis in serum samples is crucial for ensuring accurate glucose measurements. Glycolysis is the process by which glucose is broken down by cells for energy. If glycolysis occurs in a serum sample after blood collection, it can lead to a significant decrease in glucose concentration, as the cells in the sample continue to consume glucose. This reduction can result in false low readings for glucose levels in diagnostic tests, leading to misinterpretation and potential mismanagement of a patient's condition. To avoid this issue, the samples are often handled and processed carefully, sometimes using inhibitors of glycolysis or rapid cooling to minimize cellular metabolism. This practice ensures that the glucose level measured reflects the true amount present in the blood at the time of collection, which is essential for proper diagnosis and treatment. Other options, while relevant to laboratory practices, do not directly address the importance of preventing glycolysis in relation to glucose measurement accuracy.