American Society for Clinical Pathology (ASCP) Histotechnician Certification Practice Exam

What must be observed when collecting blood for trace metals analysis?

1. Use any available anticoagulant

2. Avoid the use of an anticoagulant that binds metals

3. Use glass containers exclusively

4. Collect samples in plastic tubes

The correct answer is: Avoid the use of an anticoagulant that binds metals

When collecting blood for trace metals analysis, it is essential to avoid using anticoagulants that can bind to metals. Certain anticoagulants, such as EDTA, can complex with trace metals and thus interfere with accurate measurement. If these metals are bound by the anticoagulant, the results of the trace metals analysis may be falsely low, leading to misinterpretation of the data. Using proper collection methods and containers is crucial in trace metals analysis. While the choice of container (glass or plastic) is significant, the key consideration remains the type of anticoagulant used, as this can directly impact the integrity of the sample. An understanding of how various substances interact in the testing environment is necessary to ensure that the results reflect the true levels of metals present in the blood. Thus, avoiding anticoagulants that can bind to these metals is a critical requirement in the sample collection process for trace metals analysis.